Are There Any Antibiotic Producing Bacteria in the Baker Wetlands?
Location
CoLab
Start Date
3-5-2019 12:00 PM
End Date
3-5-2019 1:15 PM
Document Type
Poster
Description
In this experiment, I am seeking to examine whether or not there are any antibiotic producing bacteria found in my soil composition. I collected my soil sample from the Baker Wetlands in Lawrence, Kansas. The soil was about 100-200 meters away from a middle-sized pond. I first concentrated my soil sample by performing serial dilutions on five different Petri dishes to obtain plates of high concentrated bacteria and low concentrated bacteria. After those were incubated, I chose twelve single colonies of bacteria and made master plates with the twelve candidates. I then tested my twelve candidates against the safe ESKAPE pathogen bacteria using the lawn of growth method. Out of my twelve candidates, one of my bacteria showed a zone of inhibition against Staphylococcus epidermis. I then made a quadrant streak of that bacteria to isolate and form a pure colony. I have not done these yet, but the last two steps I will perform are metabolic testing and sequencing of my bacteria to find at least the genus level of the organism.
Image
Are There Any Antibiotic Producing Bacteria in the Baker Wetlands?
CoLab
In this experiment, I am seeking to examine whether or not there are any antibiotic producing bacteria found in my soil composition. I collected my soil sample from the Baker Wetlands in Lawrence, Kansas. The soil was about 100-200 meters away from a middle-sized pond. I first concentrated my soil sample by performing serial dilutions on five different Petri dishes to obtain plates of high concentrated bacteria and low concentrated bacteria. After those were incubated, I chose twelve single colonies of bacteria and made master plates with the twelve candidates. I then tested my twelve candidates against the safe ESKAPE pathogen bacteria using the lawn of growth method. Out of my twelve candidates, one of my bacteria showed a zone of inhibition against Staphylococcus epidermis. I then made a quadrant streak of that bacteria to isolate and form a pure colony. I have not done these yet, but the last two steps I will perform are metabolic testing and sequencing of my bacteria to find at least the genus level of the organism.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.