Antibiotic Producing Bacteria
Location
CoLab
Start Date
3-5-2019 12:00 PM
End Date
3-5-2019 1:15 PM
Document Type
Poster
Description
Antibiotic resistance is the ability of a microbe to resist the effects of medication that used to be able to treat the microbe. In recent years the resistance towards antibiotics has increased to the point where many antibiotics are useless in today’s world. I am researching and searching for new antibiotic producing bacteria that came from a soil sample in Lawrence, KS. I did this by diluting a soil sample and placing the samples on six different agar plates. Then tested the samples against the safe relatives of highly resistant bacterial species to see what candidates showed the best zones of inhibition or “resisted” the relatives. I had eleven candidates and narrowed it down to the one that showed the best results. I have since made a quadrant streak using candidate #6 who had a large zone of inhibition against Staphylococcus epidermis. I have Gram stained, and plan to PCR test, and use metabolic testing on my candidate. Finally plan to send it off for DNA testing if the PCR test comes back with the correct amplified 16s rRNA gene. Antibiotic resistance has increased significantly and as future leaders of society we need to emphasize and expand our knowledge on antibiotics.
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Antibiotic Producing Bacteria
CoLab
Antibiotic resistance is the ability of a microbe to resist the effects of medication that used to be able to treat the microbe. In recent years the resistance towards antibiotics has increased to the point where many antibiotics are useless in today’s world. I am researching and searching for new antibiotic producing bacteria that came from a soil sample in Lawrence, KS. I did this by diluting a soil sample and placing the samples on six different agar plates. Then tested the samples against the safe relatives of highly resistant bacterial species to see what candidates showed the best zones of inhibition or “resisted” the relatives. I had eleven candidates and narrowed it down to the one that showed the best results. I have since made a quadrant streak using candidate #6 who had a large zone of inhibition against Staphylococcus epidermis. I have Gram stained, and plan to PCR test, and use metabolic testing on my candidate. Finally plan to send it off for DNA testing if the PCR test comes back with the correct amplified 16s rRNA gene. Antibiotic resistance has increased significantly and as future leaders of society we need to emphasize and expand our knowledge on antibiotics.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.