Location
CoLab, OCB 100
Start Date
25-4-2024 12:00 PM
Document Type
Poster
Description
Despite antibiotics being absolutely necessary for human and animal health, there is an ongoing crisis with a lack of discovery of new medications. Along with this crisis, many microbes are becoming resistant to currently known antibiotics. I hope with this experiment to discover new antibiotics that the microbes will not have any resistance to. This was done through a collection of soil samples in the states of Kansas and Missouri. In this experiment, I collected the sample from an area that is near to Jacoma Lake. I diluted the soil sample using 4 tubes of sterile water. Then, I incubated the microbes in screening plates. After that, I found multiple candidates that are appropriate for antibiotic screening which have a zone of inhibition. These colonies were transferred to master plates for more organization. I tested these candidates with each safe relative bacteria to figure out which candidate has antibiotic properties. I selected one candidate from 7 candidates that have a zone of inhibition and used a streak plate to use an isolated colony. Finally, this colony will be useful for further tests to figure out the genetic sequence of this antibiotic.
Image
Finding Antibiotic
CoLab, OCB 100
Despite antibiotics being absolutely necessary for human and animal health, there is an ongoing crisis with a lack of discovery of new medications. Along with this crisis, many microbes are becoming resistant to currently known antibiotics. I hope with this experiment to discover new antibiotics that the microbes will not have any resistance to. This was done through a collection of soil samples in the states of Kansas and Missouri. In this experiment, I collected the sample from an area that is near to Jacoma Lake. I diluted the soil sample using 4 tubes of sterile water. Then, I incubated the microbes in screening plates. After that, I found multiple candidates that are appropriate for antibiotic screening which have a zone of inhibition. These colonies were transferred to master plates for more organization. I tested these candidates with each safe relative bacteria to figure out which candidate has antibiotic properties. I selected one candidate from 7 candidates that have a zone of inhibition and used a streak plate to use an isolated colony. Finally, this colony will be useful for further tests to figure out the genetic sequence of this antibiotic.
Comments
The faculty mentor for this project was Melissa Beaty, Biology.