Soil Bacteria

Start Date

27-4-2023 9:00 AM

Document Type

Poster

Description

Collecting bacteria from the ground near my place to see what kind of bacteria grows within. Using serial dilution to be able to see how many colonies are in the soil, when I transfer to an agar plate and isolate it in the incubator. Once it's been in the incubator for 48 hours at room temperature, I look to see which colonies are able to look more in depth. Then challenge the colony with the safe relative and incubate them for 48 hours and see if there is a halo around the colony or not. When I have picked the colony that I'm able to look more into, that is my candidate. When I have picked my candidate, I will do a streak plate to isolate the candidate. As soon I get a single colony I'll be able to gram stain, spore, and acid fast staining to look at the candidate under a micro-scoop. Last step using PCR and Gel Electrophoersis to be able to see if the candidate has and DNA and if it does, it gets sent off to get a sequence for the candidate.

Comments

The faculty mentor for this project was Angela Consani, Biology.

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Apr 27th, 9:00 AM

Soil Bacteria

Collecting bacteria from the ground near my place to see what kind of bacteria grows within. Using serial dilution to be able to see how many colonies are in the soil, when I transfer to an agar plate and isolate it in the incubator. Once it's been in the incubator for 48 hours at room temperature, I look to see which colonies are able to look more in depth. Then challenge the colony with the safe relative and incubate them for 48 hours and see if there is a halo around the colony or not. When I have picked the colony that I'm able to look more into, that is my candidate. When I have picked my candidate, I will do a streak plate to isolate the candidate. As soon I get a single colony I'll be able to gram stain, spore, and acid fast staining to look at the candidate under a micro-scoop. Last step using PCR and Gel Electrophoersis to be able to see if the candidate has and DNA and if it does, it gets sent off to get a sequence for the candidate.