Start Date
27-4-2023 9:00 AM
Document Type
Poster
Description
In this experiment, a sample of soil was taken and many different tests were completed. The goal of this experiment was to see which microorganisms that this microorganism were inhibiting the growth of. The first that I performed was a serial dilution to get a countable plate. I then challenged my bacteria against different ESKAPE relatives. I think chose the plate that had the largest ring of inhibition against these ESKAPE relatives and named it EK9. This is the microorganism that I have been studying the entire semester. I made a streak plate and I found that that morphology of EK9 was round, entire, opaque, small, and white. I then took a colony from the streak plate and performed an acid-fast stain, a spore stain, and a gram stain. After viewing all of these stains under the oil immersion lense on the microscope, I found that EK9 was non-acid fast, gram-negative, and spore negative. I then made a Polymerase Chain Reaction and I am awaiting those results.
Image
The Discovery of Lawrence, Kansas Soil
In this experiment, a sample of soil was taken and many different tests were completed. The goal of this experiment was to see which microorganisms that this microorganism were inhibiting the growth of. The first that I performed was a serial dilution to get a countable plate. I then challenged my bacteria against different ESKAPE relatives. I think chose the plate that had the largest ring of inhibition against these ESKAPE relatives and named it EK9. This is the microorganism that I have been studying the entire semester. I made a streak plate and I found that that morphology of EK9 was round, entire, opaque, small, and white. I then took a colony from the streak plate and performed an acid-fast stain, a spore stain, and a gram stain. After viewing all of these stains under the oil immersion lense on the microscope, I found that EK9 was non-acid fast, gram-negative, and spore negative. I then made a Polymerase Chain Reaction and I am awaiting those results.
Comments
The faculty mentor for this project was Angela Consani, Biology.