Start Date

27-4-2023 9:00 AM

Document Type

Poster

Description

This experiment that I conducted is the process of soil collection and serial dilution for microbiological analysis. My sample of peaty soil was taken from Kansas City, Missouri on 1/31/2023 in cold, sunny, clear conditions from approximately 5 cm in depth, outside temperature at -3.89 degrees Celsius. I conducted a serial dilution to try to find a potential candidate. I used 1 gram of soil, some sterile water, and TSA agar plates for dilution. After incubation of 4 days, two of the plates were countable and could have had potential candidates but I did not do further experimentation with them due to having no rings of inhibition and most of the countable colonies and candidates appeared to have very similar morphology. The candidate I did pull, I named “Dr. Bailey”, a Grey’s Anatomy character. This candidate showed a strong ring of inhibition against Staphylococcus epidermis, and is the candidate I chose to study for the remainder of the semester.

Comments

The faculty mentor for this project was Angela Consani, Biology.

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Apr 27th, 9:00 AM

"Dr. Bailey"

This experiment that I conducted is the process of soil collection and serial dilution for microbiological analysis. My sample of peaty soil was taken from Kansas City, Missouri on 1/31/2023 in cold, sunny, clear conditions from approximately 5 cm in depth, outside temperature at -3.89 degrees Celsius. I conducted a serial dilution to try to find a potential candidate. I used 1 gram of soil, some sterile water, and TSA agar plates for dilution. After incubation of 4 days, two of the plates were countable and could have had potential candidates but I did not do further experimentation with them due to having no rings of inhibition and most of the countable colonies and candidates appeared to have very similar morphology. The candidate I did pull, I named “Dr. Bailey”, a Grey’s Anatomy character. This candidate showed a strong ring of inhibition against Staphylococcus epidermis, and is the candidate I chose to study for the remainder of the semester.