Discovering New Antibiotics
Start Date
27-4-2023 1:30 PM
Document Type
Poster
Description
Throughout the course of this project I have isolated a single colony of bacteria from a soil sample taken near a creek bed in Baldwin City Kansas. When this sample of soil was serially diluted I found 12 bacteria candidates that all had some form of antibiotic properties by inhibiting the growth of the other bacterial colonies near them on the agar plate. Out of my 12 candidates, I chose four to test against the safe relatives of the ESKAPE pathogens. Candidate number four showed inhibition of four of those different pathogenic relatives, which was more inhibition than the other four candidates I tested. Candidate number four is now going through further testing. It forms round, cream colored shiny colonies. I have been making streak plates to keep a pure culture, and in class we just recently put our bacteria samples through PCR testing so we can hopefully identify them soon.
Discovering New Antibiotics
Throughout the course of this project I have isolated a single colony of bacteria from a soil sample taken near a creek bed in Baldwin City Kansas. When this sample of soil was serially diluted I found 12 bacteria candidates that all had some form of antibiotic properties by inhibiting the growth of the other bacterial colonies near them on the agar plate. Out of my 12 candidates, I chose four to test against the safe relatives of the ESKAPE pathogens. Candidate number four showed inhibition of four of those different pathogenic relatives, which was more inhibition than the other four candidates I tested. Candidate number four is now going through further testing. It forms round, cream colored shiny colonies. I have been making streak plates to keep a pure culture, and in class we just recently put our bacteria samples through PCR testing so we can hopefully identify them soon.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.