Discovery of Antibiotics from the Soil
Start Date
27-4-2023 1:30 PM
Document Type
Poster
Description
Antibiotics play a crucial role in the medical field. However, antibiotic resistance has been a major challenge as microbes evolve making the antibiotic incapable of fighting bacterial infection. In spite of that, cultured bacteria from the soil have been found to have antibiotics which kill bacteria which are termed as resistant. I collected my soil sample from my backyard and used the serial dilution method to grow my bacteria. I then observed the bacteria which exhibit some resistance and aseptically transferred them to a master plate. I went ahead to test the bacteria on safe ESKAPE relatives. ESKAPE is an acronym for six highly virulent pathogens that have developed resistance against antibiotics. I then selected two potential candidates which presented distinct antibiotic characteristics. I went ahead to perform some staining as well as a Polymerase Chain of reactions to get the identity and structure of the bacteria. This data helped me see how soil microbes can play an important role in fighting antibiotic resistance and this will help fight antibiotic resistance bacterial infections.
Discovery of Antibiotics from the Soil
Antibiotics play a crucial role in the medical field. However, antibiotic resistance has been a major challenge as microbes evolve making the antibiotic incapable of fighting bacterial infection. In spite of that, cultured bacteria from the soil have been found to have antibiotics which kill bacteria which are termed as resistant. I collected my soil sample from my backyard and used the serial dilution method to grow my bacteria. I then observed the bacteria which exhibit some resistance and aseptically transferred them to a master plate. I went ahead to test the bacteria on safe ESKAPE relatives. ESKAPE is an acronym for six highly virulent pathogens that have developed resistance against antibiotics. I then selected two potential candidates which presented distinct antibiotic characteristics. I went ahead to perform some staining as well as a Polymerase Chain of reactions to get the identity and structure of the bacteria. This data helped me see how soil microbes can play an important role in fighting antibiotic resistance and this will help fight antibiotic resistance bacterial infections.
Comments
The faculty mentor for this project was Heather Seitz, Biology.