Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
The purpose of the experiment was to calculate the number of cultivable microbes per gram from a collected soil sample and then run screening against safe relative bacteria. Serial dilution is a fairly simple method that allows microorganisms to grow into countable colonies by diluting the solution. This entire experiment used a single gram of soil that I diluted into a bacterial broth, which I then spread onto an agar plate and incubated to see what would grow. I then was able to collect isolated colonies and transfer them to a separate “master” plate. Finally, I screened all the bacteria I was able to collect against safe relatives to see if my bacteria would inhibit them.
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Soil Project
The purpose of the experiment was to calculate the number of cultivable microbes per gram from a collected soil sample and then run screening against safe relative bacteria. Serial dilution is a fairly simple method that allows microorganisms to grow into countable colonies by diluting the solution. This entire experiment used a single gram of soil that I diluted into a bacterial broth, which I then spread onto an agar plate and incubated to see what would grow. I then was able to collect isolated colonies and transfer them to a separate “master” plate. Finally, I screened all the bacteria I was able to collect against safe relatives to see if my bacteria would inhibit them.
Comments
The faculty mentor for this project was Rachael Ott, Biology.