Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
Bacteria emergence has been an increasing problem within the medical community. With the growth and spread of new and previous infectious diseases from bacteria, it has been difficult to create sustainable antibiotics for these illnesses. With many new mutations forming with resistance to previous antibiotics, scientists are left to discover new ways to grow and create antibiotics made to fight off newer strains of these diseases. In our lab we used serial dilution to isolate a single bacterium from a soil sample and tested it for antibiotic properties. We isolated 9-13 individual candidates on a master plate and examined their reaction to the ESKAPE pathogens safe relatives. We also smeared and performed a series of stains to identify the properties our candidates had. We then performed a PCR test and a Gel Electrophoresis test for the DNA of our candidate. My candidate, Johnny, is gram-negative, non-spore forming, and not acid-fast.
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Stranger Things Chapter 6: The Candidate
Bacteria emergence has been an increasing problem within the medical community. With the growth and spread of new and previous infectious diseases from bacteria, it has been difficult to create sustainable antibiotics for these illnesses. With many new mutations forming with resistance to previous antibiotics, scientists are left to discover new ways to grow and create antibiotics made to fight off newer strains of these diseases. In our lab we used serial dilution to isolate a single bacterium from a soil sample and tested it for antibiotic properties. We isolated 9-13 individual candidates on a master plate and examined their reaction to the ESKAPE pathogens safe relatives. We also smeared and performed a series of stains to identify the properties our candidates had. We then performed a PCR test and a Gel Electrophoresis test for the DNA of our candidate. My candidate, Johnny, is gram-negative, non-spore forming, and not acid-fast.
Comments
The faculty mentor for this project was Angela Consani, Biology.