Start Date
28-4-2022 10:30 AM
Document Type
Poster
Description
The purpose of this project is to discover antibiotics or antibacterial components in a collection of Emma’s soil samples. Soil samples contain lots of bacteria, non-pathogenic and soil is an ideal environment for inhibitory compounds. This research of the soil sample is important in discovering antibiotics. On the soil sample I first checked for soil diversity and population by performing a serial dilution to obtain a countable plate of colonies, then went to create a master plate, then a challenging plate, streak plate, did a gram, spore stain, acid-fast stain. I chose my candidate ES003. Then I performed PCR testing, due to timing, the results will be available by the time I'm presenting. I will also perform Gel electrophoresis. All the results for these tests will be presented on my poster board.
Soil Pathogen
The purpose of this project is to discover antibiotics or antibacterial components in a collection of Emma’s soil samples. Soil samples contain lots of bacteria, non-pathogenic and soil is an ideal environment for inhibitory compounds. This research of the soil sample is important in discovering antibiotics. On the soil sample I first checked for soil diversity and population by performing a serial dilution to obtain a countable plate of colonies, then went to create a master plate, then a challenging plate, streak plate, did a gram, spore stain, acid-fast stain. I chose my candidate ES003. Then I performed PCR testing, due to timing, the results will be available by the time I'm presenting. I will also perform Gel electrophoresis. All the results for these tests will be presented on my poster board.
Comments
The faculty mentor for this project was Angela Consani, Biology .