Bacterial Properties of Soil from Prairie Village, KS
Location
CoLab, OCB 100
Start Date
27-4-2018 9:00 AM
Document Type
Poster
Description
Due to antibiotic resistance, existing antibiotics are losing their efficacy. Antibiotic resistance is said the most significant challenge facing humans today and because of this, new antibiotics are needing to be discovered in the soil. The purpose of my research project is to isolate bacteria from a soil sample and identify new sources of antibiotics. A loam soil sample was collected in Prairie Village, Kansas. A master plate of twelve unique colonies was plated on 50% TSA plates from the dilution plates. All twelve colonies were tested for their antibiotic resistance properties against Staphylococcus epidermidis, Enterobacter aerogens, Enterococcus faecalis, Escherichia coli, Pseudomonas putida, and Bacillus subtilis. Of the twelve unique colonies, only one bacteria colony showed antibiotic resistance to both Enterobacter aerogens and Bacillus subtilis. This colony is off-white, filamentous and flat. The bacterial candidate was characterized and identified through sequencing, staining, and other testing methods.
Image
Bacterial Properties of Soil from Prairie Village, KS
CoLab, OCB 100
Due to antibiotic resistance, existing antibiotics are losing their efficacy. Antibiotic resistance is said the most significant challenge facing humans today and because of this, new antibiotics are needing to be discovered in the soil. The purpose of my research project is to isolate bacteria from a soil sample and identify new sources of antibiotics. A loam soil sample was collected in Prairie Village, Kansas. A master plate of twelve unique colonies was plated on 50% TSA plates from the dilution plates. All twelve colonies were tested for their antibiotic resistance properties against Staphylococcus epidermidis, Enterobacter aerogens, Enterococcus faecalis, Escherichia coli, Pseudomonas putida, and Bacillus subtilis. Of the twelve unique colonies, only one bacteria colony showed antibiotic resistance to both Enterobacter aerogens and Bacillus subtilis. This colony is off-white, filamentous and flat. The bacterial candidate was characterized and identified through sequencing, staining, and other testing methods.
Comments
The faculty supervisor for this project was Jon Kniss, Biology.