Cain's Farm Potential Antibiotic
Location
CoLab, OCB 100
Start Date
27-4-2018 9:00 AM
Document Type
Poster
Description
The soil sample I collected was taken from a family farm located in Quenemo, Kansas. I have over fifteen years worth of priceless memories from that farm and most all of them involve being covered in dirt. So naturally, when I realized I needed to collect a soil sample from which to research this semester, I chose to collect a soil sample from the Cain’s farm. The sample was collected from the surface of the ground off to the side of an old barn. After gathering the soil, I performed a serial dilution in the lab in order to obtain a less concentrated sample to create a streak plate in hopes of finding potential candidates. Through this method I was able to isolate seventeen potential candidates and place them on two master plates. Each candidate grew with a slightly different morphology; however, the large majority of them were either round or slightly irregular with a filamentous margin. Only one candidate took over an entire master plate, rendering it useless. I was able to use my second master plate to test nine potential candidates against each of the five ESKAPE pathogens in hopes of finding a candidate that inhibited growth. Through this process I successfully found a candidate (Cain’s Farm) that inhibited the growth of E. coli.
Image
Cain's Farm Potential Antibiotic
CoLab, OCB 100
The soil sample I collected was taken from a family farm located in Quenemo, Kansas. I have over fifteen years worth of priceless memories from that farm and most all of them involve being covered in dirt. So naturally, when I realized I needed to collect a soil sample from which to research this semester, I chose to collect a soil sample from the Cain’s farm. The sample was collected from the surface of the ground off to the side of an old barn. After gathering the soil, I performed a serial dilution in the lab in order to obtain a less concentrated sample to create a streak plate in hopes of finding potential candidates. Through this method I was able to isolate seventeen potential candidates and place them on two master plates. Each candidate grew with a slightly different morphology; however, the large majority of them were either round or slightly irregular with a filamentous margin. Only one candidate took over an entire master plate, rendering it useless. I was able to use my second master plate to test nine potential candidates against each of the five ESKAPE pathogens in hopes of finding a candidate that inhibited growth. Through this process I successfully found a candidate (Cain’s Farm) that inhibited the growth of E. coli.
Comments
The faculty supervisor for this project was Heather Seitz, Biology.