Roeland Park Soil Bacterial Analysis for Antimicrobial Products
Location
CoLab, OCB 100
Start Date
27-4-2018 9:00 AM
Document Type
Poster
Description
There are hundreds of pathogen species that are known, but there are six major pathogens that are antibiotic resistant. Looking for an antibiotic that inhibits any of these pathogens is a very difficult project and in order to find an inhibitor, testing has to occur. In this research the bacteria was obtained from a soil sample. When looking at possible soil samples, it was thought that there might be numerous amounts of bacteria in the soil surrounding a tree. If the soil sample was taken from an area close to a tree, the levels of oxygen in the soil would be higher than normal. Due to a high level of oxygen, the amount of bacteria would increase because there are many kinds of bacteria that strive in aerobic environments. By using a series of dilution methods, possible inhibitor candidates were found and tested against five of the six ESKAPE pathogen relatives. After the dilutions were done, there were a total of sixteen possible candidates. Once a positive candidate was found, identification was done through staining as well as genetic and metabolic testing on a pure culture to determine the specifics of the bacteria. After the positive candidate is identified it will be further observed in a chemistry environment with the intention of creating an antibiotic that inhibits the growth of one of the six major antibiotic resistant pathogens.
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Roeland Park Soil Bacterial Analysis for Antimicrobial Products
CoLab, OCB 100
There are hundreds of pathogen species that are known, but there are six major pathogens that are antibiotic resistant. Looking for an antibiotic that inhibits any of these pathogens is a very difficult project and in order to find an inhibitor, testing has to occur. In this research the bacteria was obtained from a soil sample. When looking at possible soil samples, it was thought that there might be numerous amounts of bacteria in the soil surrounding a tree. If the soil sample was taken from an area close to a tree, the levels of oxygen in the soil would be higher than normal. Due to a high level of oxygen, the amount of bacteria would increase because there are many kinds of bacteria that strive in aerobic environments. By using a series of dilution methods, possible inhibitor candidates were found and tested against five of the six ESKAPE pathogen relatives. After the dilutions were done, there were a total of sixteen possible candidates. Once a positive candidate was found, identification was done through staining as well as genetic and metabolic testing on a pure culture to determine the specifics of the bacteria. After the positive candidate is identified it will be further observed in a chemistry environment with the intention of creating an antibiotic that inhibits the growth of one of the six major antibiotic resistant pathogens.