Isolation and Characterization of Antimicrobial Compounds Produced by Pseudomonas
Location
CoLab, COM 402
Start Date
30-4-2026 5:30 PM
Document Type
Poster
Description
Antibiotic resistance represents a growing global health crisis, highlighting the urgent need for novel antimicrobial agents, particularly those effective against Gram-negative pathogens. As part of an effort to identify new antibiotics, we investigated antibacterial compounds produced by a putative Pseudomonas strain previously isolated from soil by a microbiology student at Johnson County Community College. This isolate demonstrated inhibitory activity against Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas putida, Enterobacter aerogenes, and Acinetobacter baylyi during competitive growth assays. Crude extracts from the isolate inhibited the growth of S. epidermidis, E. faecalis, and E. coli in broth culture. Antimicrobial compounds were isolated and purified using column chromatography and high-performance liquid chromatography (HPLC), and fractions were analyzed by HPLC–mass spectrometry (HPLC-MS). These findings contribute to the ongoing search for bioactive secondary metabolites with potential therapeutic applications.
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Isolation and Characterization of Antimicrobial Compounds Produced by Pseudomonas
CoLab, COM 402
Antibiotic resistance represents a growing global health crisis, highlighting the urgent need for novel antimicrobial agents, particularly those effective against Gram-negative pathogens. As part of an effort to identify new antibiotics, we investigated antibacterial compounds produced by a putative Pseudomonas strain previously isolated from soil by a microbiology student at Johnson County Community College. This isolate demonstrated inhibitory activity against Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas putida, Enterobacter aerogenes, and Acinetobacter baylyi during competitive growth assays. Crude extracts from the isolate inhibited the growth of S. epidermidis, E. faecalis, and E. coli in broth culture. Antimicrobial compounds were isolated and purified using column chromatography and high-performance liquid chromatography (HPLC), and fractions were analyzed by HPLC–mass spectrometry (HPLC-MS). These findings contribute to the ongoing search for bioactive secondary metabolites with potential therapeutic applications.
Comments
The faculty mentor for this project was Melanie Harvey.