Location
CoLab, COM 100
Start Date
1-5-2025 6:45 PM
Document Type
Poster
Description
The goal of this research project is to find a microbe in soil that has antimicrobial properties against the safe relatives of pathogens. I started by taking a serial dilution of soil in my front yard, culturing that, and looking at what grew from it. From there I chose 12 candidates that showed inhibition and further grew them on a masterplate. The 12 promising bacterial candidates were screened against 6 ESKAPE pathogen safe relatives. The single bacteria which had the most inhibition was then used in a quadrant streak to produce isolated, pure colonies. Metabolic testing, PCR, and gel electrophoresis were used to determine the identity of the candidate.
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Finding Antimicrobials in Soil
CoLab, COM 100
The goal of this research project is to find a microbe in soil that has antimicrobial properties against the safe relatives of pathogens. I started by taking a serial dilution of soil in my front yard, culturing that, and looking at what grew from it. From there I chose 12 candidates that showed inhibition and further grew them on a masterplate. The 12 promising bacterial candidates were screened against 6 ESKAPE pathogen safe relatives. The single bacteria which had the most inhibition was then used in a quadrant streak to produce isolated, pure colonies. Metabolic testing, PCR, and gel electrophoresis were used to determine the identity of the candidate.
Comments
The faculty mentor for this project was Melissa Beaty, Biology.