Location
CoLab, OCB 100
Start Date
25-4-2024 10:30 AM
Document Type
Poster
Description
“Johnny 5” is the working name of a bacteria collected by a JCCC microbiology student. It has previously been shown to inhibit tester strains of E. aerogenes, S. epi, E. coli, E. faecalis, B. subtilis, and M. smegmatis when grown competitively. Chemical extracts taken from these bacteria inhibit tester strains of both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatographic methods. The separated fractions were tested for antimicrobial activity in broth. The results will be discussed.
Image
Extraction, Separation, and Testing of Antimicrobial Compounds Produced by a Pseudomonas Bacteria
CoLab, OCB 100
“Johnny 5” is the working name of a bacteria collected by a JCCC microbiology student. It has previously been shown to inhibit tester strains of E. aerogenes, S. epi, E. coli, E. faecalis, B. subtilis, and M. smegmatis when grown competitively. Chemical extracts taken from these bacteria inhibit tester strains of both E. coli and S. epi when grown in broth with the extract. This research explored the separation and isolation of components of the extract mixture using chromatographic methods. The separated fractions were tested for antimicrobial activity in broth. The results will be discussed.
Comments
The faculty mentor for this project was Melanie Harvey, Chemistry