Location

CoLab, OCB 100

Start Date

25-4-2024 6:00 PM

Document Type

Poster

Description

The purpose of this experiment was to discover potential new candidates of antibiotic producing bacteria, to combat the diminishing supply of effective antibiotics. In this experiment potential candidates with antibiotic-producing properties against the safe relatives of ESKAPE pathogens were collected from a soil sample. On January 27, 2024 a silty soil sample was collected just above a water line (top 3 inches) on the bank of the Shawnee Mission Park pond by Jamie Cunningham. This soil sample was serially diluted and grown on five 50% TSA agar plates. Potential candidates showing zones of inhibition were collected from dilution plates 10^-3, 10^-4, and 10^-5. These candidates were then grown onto a master plate and individually tested against each safe relative. One candidate taken from plate 10^-5 was shown to have antibiotic producing properties against Acinetobacter baylyi.

Comments

The faculty mentor for this project was Beverly Tanui, Biology.

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Apr 25th, 6:00 PM

Antibiotic Producing Bacteria

CoLab, OCB 100

The purpose of this experiment was to discover potential new candidates of antibiotic producing bacteria, to combat the diminishing supply of effective antibiotics. In this experiment potential candidates with antibiotic-producing properties against the safe relatives of ESKAPE pathogens were collected from a soil sample. On January 27, 2024 a silty soil sample was collected just above a water line (top 3 inches) on the bank of the Shawnee Mission Park pond by Jamie Cunningham. This soil sample was serially diluted and grown on five 50% TSA agar plates. Potential candidates showing zones of inhibition were collected from dilution plates 10^-3, 10^-4, and 10^-5. These candidates were then grown onto a master plate and individually tested against each safe relative. One candidate taken from plate 10^-5 was shown to have antibiotic producing properties against Acinetobacter baylyi.