Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
Microorganisms are microscopic living things that are all around us. Some of these microbes make us sick but they also serve as a reservoir for microorganisms that produce antibiotics. Over the last decades there has been a resurgence in antibiotic resistance challenging the healthcare field. Due to this issue growing in this field, scientists and researchers are having trouble finding new and effective antibiotics to replace the antibiotics that are no longer efficient, making us face an internationally global health issue. Antibiotic resistance is when the antibiotic resistance does not kill the species of bacteria that is designated to kill. Back in January I collected soil from my backyard where I took a gram of soil and have done research and studies ever since. I have performed protocols of dilutions and serial dilutions with this gram of soil, where I then created a master plate and transferred 13 potential candidates onto the media. I tested the 12 of my candidates that survived against 6 ESKAPES relatives pathogens. Where I found that TAE07A inhibited E. faecalis and A. baylyi. Recently I have streaked plated my final candidate TAE07A to move forward on with where I will perform some bacterial staining techniques, and have it go through a Polymerase Chain Reaction (PCR) to have multiple copies of DNA sequence for later DNA sequencing.
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A Determination to Find Antibiotics in Kansas Soil
Microorganisms are microscopic living things that are all around us. Some of these microbes make us sick but they also serve as a reservoir for microorganisms that produce antibiotics. Over the last decades there has been a resurgence in antibiotic resistance challenging the healthcare field. Due to this issue growing in this field, scientists and researchers are having trouble finding new and effective antibiotics to replace the antibiotics that are no longer efficient, making us face an internationally global health issue. Antibiotic resistance is when the antibiotic resistance does not kill the species of bacteria that is designated to kill. Back in January I collected soil from my backyard where I took a gram of soil and have done research and studies ever since. I have performed protocols of dilutions and serial dilutions with this gram of soil, where I then created a master plate and transferred 13 potential candidates onto the media. I tested the 12 of my candidates that survived against 6 ESKAPES relatives pathogens. Where I found that TAE07A inhibited E. faecalis and A. baylyi. Recently I have streaked plated my final candidate TAE07A to move forward on with where I will perform some bacterial staining techniques, and have it go through a Polymerase Chain Reaction (PCR) to have multiple copies of DNA sequence for later DNA sequencing.
Comments
The faculty mentor for this project was Heather Seitz, Biology.