Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
There are many types of microorganisms around us, such as bacteria and fungi, that are essential to complex organisms such as human beings and animals and are vital for survival because these microscopic organisms play an essential role in the ecosystem. However, bacteria have “bad” or disease-causing properties and good properties through antibiotics, which is an antimicrobial substance that is active against bacteria and is commonly found in microorganisms. To carry out my experiment, I collected one gram of soil from the Johnson county community college area around the Regnier center, and after following the protocols of dilution and serial dilution I created a master plate with ten potential candidates that were derived from the candidates that I chose from the agar plates. After screening the candidates using the safe relatives, that is, ESKAPE Pathogens, most of my candidates could not inhibit any antibiotic growth. Thankfully a classmate saved the day and let me use one of her samples, namely “W.M 5,” derived from the wetlands meadow in Lawrence, Kansas, that inhibited antibiotic growth when screened against Staphylococcus epidermis. A WM5 was streak plated and underwent polymerase chain reaction (PCR) to isolate the millions of copies of DNA that will be used to copy a specific sequence of DNA outside of the living cell.
Image
Antibiotics in the Soil Environment
There are many types of microorganisms around us, such as bacteria and fungi, that are essential to complex organisms such as human beings and animals and are vital for survival because these microscopic organisms play an essential role in the ecosystem. However, bacteria have “bad” or disease-causing properties and good properties through antibiotics, which is an antimicrobial substance that is active against bacteria and is commonly found in microorganisms. To carry out my experiment, I collected one gram of soil from the Johnson county community college area around the Regnier center, and after following the protocols of dilution and serial dilution I created a master plate with ten potential candidates that were derived from the candidates that I chose from the agar plates. After screening the candidates using the safe relatives, that is, ESKAPE Pathogens, most of my candidates could not inhibit any antibiotic growth. Thankfully a classmate saved the day and let me use one of her samples, namely “W.M 5,” derived from the wetlands meadow in Lawrence, Kansas, that inhibited antibiotic growth when screened against Staphylococcus epidermis. A WM5 was streak plated and underwent polymerase chain reaction (PCR) to isolate the millions of copies of DNA that will be used to copy a specific sequence of DNA outside of the living cell.
Comments
The faculty mentor for this project was Heather Seitz, Biology.