Start Date
27-4-2023 10:30 AM
Document Type
Poster
Description
Many microorganisms like bacteria and viruses develop resistance to antibiotics; which are produced to kill them. When a microbe has tested and proved sustainable against each and every antibiotic, what's next? In my Microbiology lab course I have been testing candidates for antibiotic producing properties. I have taken a soil sample from my backyard and have isolated colonies of microbes to test against different tester strains of bacteria. I have serially diluted my soil to spread to Agar plates to incubate and grow. From there I carefully selected eight promising candidates to further incubate on a master plate. I then selected four candidates from those eight to be tested against prominent bacterial tester strains. One specific candidate inhibited the growth of the following strains: Enterococcus Faecalis, Acinetobacter baylyi, Enterobacter aerogenes, Escherichia Coli, and Pseudomonas putida. I have continued growing this candidate weekly on streak plates to maintain a pure culture. Most recently I advanced to a PCR test of my bacteria to amplify the RNA genes of that candidate. I have kept a record collection of my data along with the materials and methods that I've used along the process to be submitted to Tiny Earth, which will conclude with my overall results of my analysis.
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Creating Antibiotic Producing Candidates From Soil
Many microorganisms like bacteria and viruses develop resistance to antibiotics; which are produced to kill them. When a microbe has tested and proved sustainable against each and every antibiotic, what's next? In my Microbiology lab course I have been testing candidates for antibiotic producing properties. I have taken a soil sample from my backyard and have isolated colonies of microbes to test against different tester strains of bacteria. I have serially diluted my soil to spread to Agar plates to incubate and grow. From there I carefully selected eight promising candidates to further incubate on a master plate. I then selected four candidates from those eight to be tested against prominent bacterial tester strains. One specific candidate inhibited the growth of the following strains: Enterococcus Faecalis, Acinetobacter baylyi, Enterobacter aerogenes, Escherichia Coli, and Pseudomonas putida. I have continued growing this candidate weekly on streak plates to maintain a pure culture. Most recently I advanced to a PCR test of my bacteria to amplify the RNA genes of that candidate. I have kept a record collection of my data along with the materials and methods that I've used along the process to be submitted to Tiny Earth, which will conclude with my overall results of my analysis.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.