Start Date
27-4-2023 10:30 AM
Document Type
Poster
Description
This project aimed at gathering data on genetic mutations as a contribution to the collaborative research study which originated at UC Davis, Design2Data (D2D). Through the use of a protein-modeling software program, FoldIt Standalone, predictions were made about the efficacy of a selected mutation to a DNA sequence responsible for the production of the enzyme Beta-Glucosidase B (Bgl B). The mutation was successfully carried out in the laboratory by means of Kunkel mutagenesis. The DNA was then activated with the help of BL21 E. coli cells, resulting in the successful production of the mutated Bgl B enzyme. Through analysis of the mutated enzyme, we will be able to provide real world data which will be used to improve the accuracy of protein-modeling algorithms for programs such as FoldIt Standalone. This data, along with that collected by other members of the D2D community, will give insight into the functionality of Bgl B while providing a better understanding of modern, technological approaches to genetic engineering.
Image
A Genetic Mutation of Beta-Glucosidase B
This project aimed at gathering data on genetic mutations as a contribution to the collaborative research study which originated at UC Davis, Design2Data (D2D). Through the use of a protein-modeling software program, FoldIt Standalone, predictions were made about the efficacy of a selected mutation to a DNA sequence responsible for the production of the enzyme Beta-Glucosidase B (Bgl B). The mutation was successfully carried out in the laboratory by means of Kunkel mutagenesis. The DNA was then activated with the help of BL21 E. coli cells, resulting in the successful production of the mutated Bgl B enzyme. Through analysis of the mutated enzyme, we will be able to provide real world data which will be used to improve the accuracy of protein-modeling algorithms for programs such as FoldIt Standalone. This data, along with that collected by other members of the D2D community, will give insight into the functionality of Bgl B while providing a better understanding of modern, technological approaches to genetic engineering.
Comments
The faculty mentor for this project was Heather Seitz, Biology.