Start Date
27-4-2023 10:30 AM
Document Type
Poster
Description
The lack of interest in the ability to efficiently create never-before-seen mutations in proteins for novel therapeutics, and industrial and agricultural applications has sent an alarming risk for uncertainty for when these mutations occur. Luckily, JCCC has partnered with Design 2 Data (D2D), which goal is to facilitate "academic crowd-sourcing" to rapidly address protein design questions that would normally take isolated labs decades to answer. Through this partnership, the protein called beta-glucosidase B is used as well as a chosen mutation D279R. This was done by mutation an enzyme in BglB's to predict the impact of stability through a software called FoldIt, which predicted better enzyme function due to its decent stability. Further testing of the mutant enzyme will be done to predict the impact of the mutation on enzyme activity and stability.
Image
The Affects of Mutation D279R in the Beta-Glucosidase B Protein
The lack of interest in the ability to efficiently create never-before-seen mutations in proteins for novel therapeutics, and industrial and agricultural applications has sent an alarming risk for uncertainty for when these mutations occur. Luckily, JCCC has partnered with Design 2 Data (D2D), which goal is to facilitate "academic crowd-sourcing" to rapidly address protein design questions that would normally take isolated labs decades to answer. Through this partnership, the protein called beta-glucosidase B is used as well as a chosen mutation D279R. This was done by mutation an enzyme in BglB's to predict the impact of stability through a software called FoldIt, which predicted better enzyme function due to its decent stability. Further testing of the mutant enzyme will be done to predict the impact of the mutation on enzyme activity and stability.
Comments
The faculty mentor for this project was Heather Seitz, Biology.