Antibody Ariel
Start Date
27-4-2023 1:30 PM
Document Type
Poster
Description
The world is testing different antibodies that are found to someday help the fight against superbugs. In a JCCC Microbiology Laboratory class taught by Professor Angela Consani we would begin this process simply with a ziplock bag of soil. This soil sample was taken from Overland Park, KS, and was serial diluted to start testing. When put on a 50% Tryptic Soy Agar plate, if they showed any zone of inhibition, they could now be considered a candidate. I started with 10 but one candidate named Mulan had to be discontinued because she was contaminating the plate, growing mold. With the remaining candidates, I tested them against the ESKAPE pathogens, looking and measuring the zone of inhibition again. With further testing I chose to continue my project with candidate Ariel. I then was able to gram and spore stain the sample to be viewed under the microscope. Learning new techniques allowed me to figure out that the sample is a very small, non-spore forming organism. She will now go through the PCR process to hopefully find her genetic makeup.
Antibody Ariel
The world is testing different antibodies that are found to someday help the fight against superbugs. In a JCCC Microbiology Laboratory class taught by Professor Angela Consani we would begin this process simply with a ziplock bag of soil. This soil sample was taken from Overland Park, KS, and was serial diluted to start testing. When put on a 50% Tryptic Soy Agar plate, if they showed any zone of inhibition, they could now be considered a candidate. I started with 10 but one candidate named Mulan had to be discontinued because she was contaminating the plate, growing mold. With the remaining candidates, I tested them against the ESKAPE pathogens, looking and measuring the zone of inhibition again. With further testing I chose to continue my project with candidate Ariel. I then was able to gram and spore stain the sample to be viewed under the microscope. Learning new techniques allowed me to figure out that the sample is a very small, non-spore forming organism. She will now go through the PCR process to hopefully find her genetic makeup.
Comments
The faculty mentor for this project was Angela Consani, Biology.