Antibiotics in Soil

Start Date

27-4-2023 1:30 PM

Document Type

Poster

Description

Over the semester I have been testing candidates for antibiotic resistance. I obtained a soil sample, then serially diluted the sample and placed the dilutions on agar plates so that candidates could grow. I then took the candidates that show inhibition to the other bacteria on the plate and made a master plate. I finally tested these candidates against E.S.K.A.P.E relatives. Screening against these relatives will give us an idea if the candidate will show inhibition against serious pathogens that we cannot safely test against in this class. I think this research is important because antibiotic resistance will always be an issue and we need to try to be one step ahead. As a class we are all searching for a new antibiotic, if even one of us can find a good candidate more research could be done and we could have a new antibiotic on our hands. A few of my candidates have shown inhibition towards E.S.K.A.P.E relatives, but I have chosen my candidate LA9 to move forward with. LA9 show inhibition against Enterococcus faecium, Staphylococcus aureus, and Escherichia coli.

Comments

The faculty mentor for this project was Heather Seitz, Biology.

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Apr 27th, 1:30 PM

Antibiotics in Soil

Over the semester I have been testing candidates for antibiotic resistance. I obtained a soil sample, then serially diluted the sample and placed the dilutions on agar plates so that candidates could grow. I then took the candidates that show inhibition to the other bacteria on the plate and made a master plate. I finally tested these candidates against E.S.K.A.P.E relatives. Screening against these relatives will give us an idea if the candidate will show inhibition against serious pathogens that we cannot safely test against in this class. I think this research is important because antibiotic resistance will always be an issue and we need to try to be one step ahead. As a class we are all searching for a new antibiotic, if even one of us can find a good candidate more research could be done and we could have a new antibiotic on our hands. A few of my candidates have shown inhibition towards E.S.K.A.P.E relatives, but I have chosen my candidate LA9 to move forward with. LA9 show inhibition against Enterococcus faecium, Staphylococcus aureus, and Escherichia coli.