Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
This project involves a bacteria found in my backyard in Grandview, Missouri. The purpose of my project is to find a microorganism that produces an antibiotic or resemblance of antibiotic properties. After going through a soil dilution to create several series of master plates, I was able to narrow down a few candidates which seemed to show good inhibition for further investigation. From my final master plate I picked a single candidate that I chose to do a gram stain on, as well as a streak plate, and tested against other pathogens. My candidate was negative shown through the gram stain, and from looking through the microscope at this microorganism it was coccus shaped. My streak plate was able to isolate several colonies for further investigation that we are now using to do a PCR screening of. My microorganism held up well against pathogens like E. coli, E. faecalis, and S. epidermis. In class we are still further investigating and testing our pathogens, but this is as far as we have gotten. However it seems as if my microorganism could be one step closer to fighting this ongoing battle of antibiotic resistance.
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Microorganism in Depth
This project involves a bacteria found in my backyard in Grandview, Missouri. The purpose of my project is to find a microorganism that produces an antibiotic or resemblance of antibiotic properties. After going through a soil dilution to create several series of master plates, I was able to narrow down a few candidates which seemed to show good inhibition for further investigation. From my final master plate I picked a single candidate that I chose to do a gram stain on, as well as a streak plate, and tested against other pathogens. My candidate was negative shown through the gram stain, and from looking through the microscope at this microorganism it was coccus shaped. My streak plate was able to isolate several colonies for further investigation that we are now using to do a PCR screening of. My microorganism held up well against pathogens like E. coli, E. faecalis, and S. epidermis. In class we are still further investigating and testing our pathogens, but this is as far as we have gotten. However it seems as if my microorganism could be one step closer to fighting this ongoing battle of antibiotic resistance.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.