Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
The search for new antibiotic continues at JCCC! In this study, a loam soil sample was taken from Heritage Dog Park (38 degrees, 51', 28" N; 94 degrees, 46', 22" W) to identify and isolate bacterial strains that produce antibiotics against ESKAPE pathogens. After an initial screen on 50% TSA agar plates, the candidate bacteria, SAM3, was selected for further evaluation based on a zones of inhibition for other soil bacteria. A Gram-stain identified SAM3 as a gram-negative bacillus rod, and its pattern of hemolysis on a blood agar plate indicated that it is beta-hemolytic. Based on visual inspection, there is a strong indication that SAM3 is also spore-forming. Its colony morphology is rhizoid with irregular edges, it grows moderately deeply into the agar and it's a light cream color. SAM3 had significant zones of inhibition for E. coli, A. baylyi and P. putida, and had strong zones of inhibition for E. faecalis and S. epidermidis. The 16s rRNA from this microbe has been isolated and sent off to be sequenced as further research is conducted.
Image
Isolation of Antibiotic-Producing Bacteria from Soil Sample
The search for new antibiotic continues at JCCC! In this study, a loam soil sample was taken from Heritage Dog Park (38 degrees, 51', 28" N; 94 degrees, 46', 22" W) to identify and isolate bacterial strains that produce antibiotics against ESKAPE pathogens. After an initial screen on 50% TSA agar plates, the candidate bacteria, SAM3, was selected for further evaluation based on a zones of inhibition for other soil bacteria. A Gram-stain identified SAM3 as a gram-negative bacillus rod, and its pattern of hemolysis on a blood agar plate indicated that it is beta-hemolytic. Based on visual inspection, there is a strong indication that SAM3 is also spore-forming. Its colony morphology is rhizoid with irregular edges, it grows moderately deeply into the agar and it's a light cream color. SAM3 had significant zones of inhibition for E. coli, A. baylyi and P. putida, and had strong zones of inhibition for E. faecalis and S. epidermidis. The 16s rRNA from this microbe has been isolated and sent off to be sequenced as further research is conducted.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.