Antibiotic Producing Bacteria
Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
The purpose of this experiment was to isolate antibiotic producing bacteria from a soil sample. This soil sample was a loam sample collected from a residential lawn in Kansas City Missouri. After diluting the soil sample, the candidates were spread onto eight 50% TSA agar plates and incubated for a week. The plates with higher dilution exhibited countable colonies and was used for further testing. Plate 10-5 dilution was selected for use having 178 countable colonies, from those 178, 12 antibiotic producing candidates exhibited clear zones of inhibition. These candidates were collected and transferred to a master plate and labeled 1-12 and incubated for a week. A new master plate was configured once again, narrowing down the 12 candidates into five that showed clear zones of inhibition. These five candidates were then transferred to the new master plate and labeled using their original number from original master plate and incubated for a week. These candidates were numbers 4, 5, 8, 9, and 12. It was observed that candidate 4 had irregular texture, and erose margin. Candidate 5 did not exhibit growth. Candidate 9 grew into a large colony, light beige in color with a matte appearance and irregular margin. Candidate 12 was glossy in appearance, light yellow in color, with an irregular margin. These candidates were then tested for resistance against ESKAPE pathogens Enterococcus faecium, Staphylococcus aureus, Acinetobacter baumannii, Enterobacter, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Candidate 4 showed antibiotic resistance against all ESKAPE pathogens.
Antibiotic Producing Bacteria
The purpose of this experiment was to isolate antibiotic producing bacteria from a soil sample. This soil sample was a loam sample collected from a residential lawn in Kansas City Missouri. After diluting the soil sample, the candidates were spread onto eight 50% TSA agar plates and incubated for a week. The plates with higher dilution exhibited countable colonies and was used for further testing. Plate 10-5 dilution was selected for use having 178 countable colonies, from those 178, 12 antibiotic producing candidates exhibited clear zones of inhibition. These candidates were collected and transferred to a master plate and labeled 1-12 and incubated for a week. A new master plate was configured once again, narrowing down the 12 candidates into five that showed clear zones of inhibition. These five candidates were then transferred to the new master plate and labeled using their original number from original master plate and incubated for a week. These candidates were numbers 4, 5, 8, 9, and 12. It was observed that candidate 4 had irregular texture, and erose margin. Candidate 5 did not exhibit growth. Candidate 9 grew into a large colony, light beige in color with a matte appearance and irregular margin. Candidate 12 was glossy in appearance, light yellow in color, with an irregular margin. These candidates were then tested for resistance against ESKAPE pathogens Enterococcus faecium, Staphylococcus aureus, Acinetobacter baumannii, Enterobacter, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Candidate 4 showed antibiotic resistance against all ESKAPE pathogens.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.