Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
Antibiotic resistant bacteria is a recurring issue that we come across every single day. With that in mind, this research consists of a clay soil sample obtained from my backyard, in Blue Springs Missouri, and experimenting with it further in order to observe and identify potential bacterial candidates that are antibiotic resistant. The steps taken to note such resistance consists of taking a soil dilution from the original soil sample, transferring all potential candidates to a master plate, creating a new master plate of the notable candidates that appear to have no excessive growth, with some resistance to antibodies present, screening chosen candidates against antibiotic properties, as well as creating a streak plate of the best candidates against what are known as the ESKAPE relatives. The ESKAPE relatives that my best candidate shows effectiveness against consist of Enterobacter aerogenes, Staphylococcus epidermidis, and Acinetobacter baylyi. The ESKAPE pathogens that the candidate shows no effectiveness against consist of Escherichia coli, Enterococcus faecalis, and Pseudomonas putida. After completing these steps, one final candidate was chosen for further experiments in order to test its isolated colonies, which was the best candidate effective against most of the ESKAPE relatives mentioned above.
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Identifying Bacterial Antibodies from Soil
Antibiotic resistant bacteria is a recurring issue that we come across every single day. With that in mind, this research consists of a clay soil sample obtained from my backyard, in Blue Springs Missouri, and experimenting with it further in order to observe and identify potential bacterial candidates that are antibiotic resistant. The steps taken to note such resistance consists of taking a soil dilution from the original soil sample, transferring all potential candidates to a master plate, creating a new master plate of the notable candidates that appear to have no excessive growth, with some resistance to antibodies present, screening chosen candidates against antibiotic properties, as well as creating a streak plate of the best candidates against what are known as the ESKAPE relatives. The ESKAPE relatives that my best candidate shows effectiveness against consist of Enterobacter aerogenes, Staphylococcus epidermidis, and Acinetobacter baylyi. The ESKAPE pathogens that the candidate shows no effectiveness against consist of Escherichia coli, Enterococcus faecalis, and Pseudomonas putida. After completing these steps, one final candidate was chosen for further experiments in order to test its isolated colonies, which was the best candidate effective against most of the ESKAPE relatives mentioned above.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.