Start Date
27-4-2023 12:00 PM
Document Type
Poster
Description
The purpose behind this project is to isolate a bacteria that consists of antibiotic resistant characteristics, which is originally derived from a soil sample. This soil sample was achieved from my front yard at a temperature of 33 degrees Fahrenheit. The type of soil the bacteria is derived from is loam, having a pH of 6.5. Taking 1 gram of that soil sample, a serial dilution was performed to dilute the soil sample to help identify antibiotic producing candidates. Additional screening was done on several bacterial residents that showed to have antibiotic resistant characteristics to help further isolate those candidates and choose which one best represents antibiotic resistance. After several trials of transferring candidates by making master plates to help represent the best candidates and further isolate them, 3 candidates were then used for further screening against safe relatives of ESKAPE pathogens. In conclusion, one candidate was the most effective against almost all safe relatives, including Enterococcus faecalis, Pseudomonas putida, Enterobacter aerogenes, Escherichia coli, and finally Acinetobacter baylyi. The only safe relative it wasn’t effective against was Staphylococcus epidermidis. The other 2 candidates weren’t as effective against these safe relatives, so the candidate that was most effective against them was chosen to continue to use for further testing. This candidate is a round colony that has a convex elevation with a smooth margin, and has an orange color. Two trials of making streak plates have been made to help isolate colonies of this bacteria candidate. Further testing in the future will be made to help identify this bacterial candidate.
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Antibiotic Resisting Bacteria Derived from Loam Soil Sample
The purpose behind this project is to isolate a bacteria that consists of antibiotic resistant characteristics, which is originally derived from a soil sample. This soil sample was achieved from my front yard at a temperature of 33 degrees Fahrenheit. The type of soil the bacteria is derived from is loam, having a pH of 6.5. Taking 1 gram of that soil sample, a serial dilution was performed to dilute the soil sample to help identify antibiotic producing candidates. Additional screening was done on several bacterial residents that showed to have antibiotic resistant characteristics to help further isolate those candidates and choose which one best represents antibiotic resistance. After several trials of transferring candidates by making master plates to help represent the best candidates and further isolate them, 3 candidates were then used for further screening against safe relatives of ESKAPE pathogens. In conclusion, one candidate was the most effective against almost all safe relatives, including Enterococcus faecalis, Pseudomonas putida, Enterobacter aerogenes, Escherichia coli, and finally Acinetobacter baylyi. The only safe relative it wasn’t effective against was Staphylococcus epidermidis. The other 2 candidates weren’t as effective against these safe relatives, so the candidate that was most effective against them was chosen to continue to use for further testing. This candidate is a round colony that has a convex elevation with a smooth margin, and has an orange color. Two trials of making streak plates have been made to help isolate colonies of this bacteria candidate. Further testing in the future will be made to help identify this bacterial candidate.
Comments
The faculty mentor for this project was Matthew Ducote, Biology.