Location
OCB 100
Start Date
28-4-2022 9:00 AM
Document Type
Poster
Description
The search for novel antibiotic producing microorganisms has been launched in order to fight antibiotic resistance crisis, multiple consecutive experiments are set up as part of the entire process. As a student of microbiology I have the opportunity the participate in this research. Here in my research paper I’m presenting all the lab work I have completed in hopes of finding a potential candidate with inhibition abilities. The whole process consists of soil collection, serial dilution, candidate selection and isolation, Gram stain, and PCR. One of my major realizations is that; accuracy and precision are fundamental in conducting microbiology lab experiments. The more I advanced in my project, the less results I seemed to have and that relies on several facts that negatively affected the course of my research such as the type of soil I collected and the area it was collected from, poorly mixed water/soil solution during serial dilution…etc. I finally got to the point where I needed to adopt one of my class mates candidate. The candidate’s name is AYA010 and forms Convex, smooth, yellow colonies. The important thing that makes it a candidate is that it has shown inhibition signs to different ESKAPE relatives and it is going to be sent off for DNA sequencing after PCR reaction for more convincing evidence about what microorganism it is and how does it function.
Image
Looking For a Candidate
OCB 100
The search for novel antibiotic producing microorganisms has been launched in order to fight antibiotic resistance crisis, multiple consecutive experiments are set up as part of the entire process. As a student of microbiology I have the opportunity the participate in this research. Here in my research paper I’m presenting all the lab work I have completed in hopes of finding a potential candidate with inhibition abilities. The whole process consists of soil collection, serial dilution, candidate selection and isolation, Gram stain, and PCR. One of my major realizations is that; accuracy and precision are fundamental in conducting microbiology lab experiments. The more I advanced in my project, the less results I seemed to have and that relies on several facts that negatively affected the course of my research such as the type of soil I collected and the area it was collected from, poorly mixed water/soil solution during serial dilution…etc. I finally got to the point where I needed to adopt one of my class mates candidate. The candidate’s name is AYA010 and forms Convex, smooth, yellow colonies. The important thing that makes it a candidate is that it has shown inhibition signs to different ESKAPE relatives and it is going to be sent off for DNA sequencing after PCR reaction for more convincing evidence about what microorganism it is and how does it function.
Comments
The faculty mentor for this project was Angela Consani, Biology.