Location
OCB 100
Start Date
28-4-2022 9:00 AM
Document Type
Poster
Description
Here in the Midwest, we know that good soil is essential for many things. However, soil can unknowingly harbor dangerous microbes. For this project, each member of our class obtained a sample of soil from a chosen area and conducted a series of experiments to test for an interesting microbial organism. My soil was sampled from my garden bed and proved to have many different bacteria present. Through a series of soil tests and serial dilution, I was able to identify many organisms and better examine them on a master plate. Additionally, through various challenge plates I was able to test the organisms for antibiotic resistance and eventually found my most intriguing microbial candidate. I created a streak plate for this sample, Species G, and kept for further testing. I found that the colonies appeared orange in color, small, round, and flat shaped. The cells of the microbe when viewed under the microscope proved to be extremely small in size, bacilli shaped, gram-negative, with no spores. Further testing will be done with Species G including PCR for genome sequencing to ultimately find out what my microbial candidate is.
Image
Mr. Orange Derives From Soil
OCB 100
Here in the Midwest, we know that good soil is essential for many things. However, soil can unknowingly harbor dangerous microbes. For this project, each member of our class obtained a sample of soil from a chosen area and conducted a series of experiments to test for an interesting microbial organism. My soil was sampled from my garden bed and proved to have many different bacteria present. Through a series of soil tests and serial dilution, I was able to identify many organisms and better examine them on a master plate. Additionally, through various challenge plates I was able to test the organisms for antibiotic resistance and eventually found my most intriguing microbial candidate. I created a streak plate for this sample, Species G, and kept for further testing. I found that the colonies appeared orange in color, small, round, and flat shaped. The cells of the microbe when viewed under the microscope proved to be extremely small in size, bacilli shaped, gram-negative, with no spores. Further testing will be done with Species G including PCR for genome sequencing to ultimately find out what my microbial candidate is.
Comments
The faculty mentor for this project was Angela Consani, Biology.