Location
OCB 100
Start Date
28-4-2022 1:30 PM
Document Type
Poster
Description
Since being discovered, antibiotics have been frequently used on patients in the medical field. Due to their prevalent use, there is a rising threat of antibiotic resistance. The purpose of this experiment is to determine a possible candidate (or possible candidates) for antibiotic testing. This experiment began with the serial dilution of a collected soil sample. The serial dilutions were added to plates that contained an agar to encourage bacterial growth. Bacterial colonies that showed possible zones of inhibition were then selected for testing as possible antibiotic candidates. Once adequate candidates were discovered, they were tested against six relatives of ESKAPE pathogens. Of the nine candidates selected and tested, one showed a zone of inhibition against a gram-negative and a gram-positive ESKAPE relative. The last part of this experiment will incorporate further metabolic and DNA sequence testing of this candidate as a possible antibiotic.
Image
Antibiotic Screening
OCB 100
Since being discovered, antibiotics have been frequently used on patients in the medical field. Due to their prevalent use, there is a rising threat of antibiotic resistance. The purpose of this experiment is to determine a possible candidate (or possible candidates) for antibiotic testing. This experiment began with the serial dilution of a collected soil sample. The serial dilutions were added to plates that contained an agar to encourage bacterial growth. Bacterial colonies that showed possible zones of inhibition were then selected for testing as possible antibiotic candidates. Once adequate candidates were discovered, they were tested against six relatives of ESKAPE pathogens. Of the nine candidates selected and tested, one showed a zone of inhibition against a gram-negative and a gram-positive ESKAPE relative. The last part of this experiment will incorporate further metabolic and DNA sequence testing of this candidate as a possible antibiotic.
Comments
The faculty mentor for this project was Melissa Beaty, Biology.