Location
OCB 100
Start Date
28-4-2022 1:30 PM
Document Type
Poster
Description
The purpose of this study is to isolate a sample of loamy soil, collected in Kansas City, Kansas in my backyard, to test for the possible presence of bacteria that could be produce an antibiotic. Most antibiotics are commonly found in soil samples, thus, a new antibiotic could be discovered. My soil sample collected was used in series of serial dilution, then potential colonies formed from serial dilution were transferred to a master plate that showed inhibition, then to screening plates with 6 safe ESKAPE pathogen relatives. From here, I concluded which candidates inhibited growth of these ESKAPE pathogens and further purified them, 4 times, by streak plating one. The streak plate of the potential candidate that inhibited Acinetobacter baylyi was done to find individual colonies that will give me the bacteria DNA, which I would then later use the polymerase chain reaction test to give me multiple copies of the sequence of DNA found in my candidate.
Image
Antibiotic Screening From Soil
OCB 100
The purpose of this study is to isolate a sample of loamy soil, collected in Kansas City, Kansas in my backyard, to test for the possible presence of bacteria that could be produce an antibiotic. Most antibiotics are commonly found in soil samples, thus, a new antibiotic could be discovered. My soil sample collected was used in series of serial dilution, then potential colonies formed from serial dilution were transferred to a master plate that showed inhibition, then to screening plates with 6 safe ESKAPE pathogen relatives. From here, I concluded which candidates inhibited growth of these ESKAPE pathogens and further purified them, 4 times, by streak plating one. The streak plate of the potential candidate that inhibited Acinetobacter baylyi was done to find individual colonies that will give me the bacteria DNA, which I would then later use the polymerase chain reaction test to give me multiple copies of the sequence of DNA found in my candidate.
Comments
The faculty mentor for this project was Matt Ducote, Biology.