Growing Mary
Location
CoLab
Start Date
3-5-2019 9:00 AM
End Date
3-5-2019 10:15 AM
Document Type
Poster
Description
The purpose of this testing is to try and find a new bacterium for a new antibiotic to help in the growing problem of antibiotic resistance. My soil sample was collected from a front yard in Overland Park. After completing my serial dilutions, 12 colonies were plated on a master plate for potential candidates. Only five colonies grew on the master plate. After screening against the six ESKAPE pathogens no zones of inhibition were found. I adopted a candidate from a classmate Julia, she named this candidate Mary. Mary came from silty soil collected from an apartment complex in Overland Park. A zone of inhibition was found when screened against P. putia. Mary’s morphology is white, round, convex and has a matte finish.
Image
Growing Mary
CoLab
The purpose of this testing is to try and find a new bacterium for a new antibiotic to help in the growing problem of antibiotic resistance. My soil sample was collected from a front yard in Overland Park. After completing my serial dilutions, 12 colonies were plated on a master plate for potential candidates. Only five colonies grew on the master plate. After screening against the six ESKAPE pathogens no zones of inhibition were found. I adopted a candidate from a classmate Julia, she named this candidate Mary. Mary came from silty soil collected from an apartment complex in Overland Park. A zone of inhibition was found when screened against P. putia. Mary’s morphology is white, round, convex and has a matte finish.
Comments
The faculty supervisor for this project was Heather Seitz, Biology.