Searching for Antibiotic Producing Bacteria
Location
CoLab
Start Date
3-5-2019 1:30 PM
End Date
3-5-2019 2:45 PM
Document Type
Poster
Description
The goal of this research is to find new antibiotic producing bacteria to use against antibiotic resistant strains. The search began by collecting soil and doing a serial dilution. After dilution twelve potential candidates were placed in a master plate. After creating individual screening of the candidates against the safe relatives of the ESKAPE pathogens one candidate was chosen for genetic sequencing of the 16s rRNA gene and metabolic testing. To do so a quadrant streak was made to isolate a colony. Sequencing will allow us to at least find out the genus of the antibiotic producing candidate. Metabolic testing will be used to confirm results of the PCR sequencing.
Image
Searching for Antibiotic Producing Bacteria
CoLab
The goal of this research is to find new antibiotic producing bacteria to use against antibiotic resistant strains. The search began by collecting soil and doing a serial dilution. After dilution twelve potential candidates were placed in a master plate. After creating individual screening of the candidates against the safe relatives of the ESKAPE pathogens one candidate was chosen for genetic sequencing of the 16s rRNA gene and metabolic testing. To do so a quadrant streak was made to isolate a colony. Sequencing will allow us to at least find out the genus of the antibiotic producing candidate. Metabolic testing will be used to confirm results of the PCR sequencing.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.