Screening for Antibodies Found in Dirt of Backyard
Location
CoLab
Start Date
3-5-2019 10:30 AM
End Date
3-5-2019 11:45 AM
Document Type
Poster
Description
The purpose of this experiment was to test for any antibiotic producing bacteria from surface level dirt in the backyard of a suburban neighborhood. From the dirt, serial dilutions were done to test for at least twelve zones of inhibition which were transferred into master plates to separate the bacteria. All twelve of the bacteria from the master plate were screened against the following tester strains: Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Acinetobacter baylyi, Pseudomonas putida, Enterobacter aerogenes. The first bacteria from the master plate was found to have antibiotic resistance to E. coli, A. baylyi, P. putida, E. aerogenes. A quadrant streak was performed on the bacteria to isolate it. From this quadrant streak, we are able to do metabolic testing and sequencing to know the genus level of organism was performed.
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Screening for Antibodies Found in Dirt of Backyard
CoLab
The purpose of this experiment was to test for any antibiotic producing bacteria from surface level dirt in the backyard of a suburban neighborhood. From the dirt, serial dilutions were done to test for at least twelve zones of inhibition which were transferred into master plates to separate the bacteria. All twelve of the bacteria from the master plate were screened against the following tester strains: Enterococcus faecalis, Staphylococcus epidermidis, Escherichia coli, Acinetobacter baylyi, Pseudomonas putida, Enterobacter aerogenes. The first bacteria from the master plate was found to have antibiotic resistance to E. coli, A. baylyi, P. putida, E. aerogenes. A quadrant streak was performed on the bacteria to isolate it. From this quadrant streak, we are able to do metabolic testing and sequencing to know the genus level of organism was performed.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.