Discovery and Analysis of Antibiotic Producing Bacteria from Locally Sourced Soil
Location
CoLab
Start Date
3-5-2019 12:00 PM
End Date
3-5-2019 1:15 PM
Document Type
Poster
Description
Locally sourced soil was extracted in order to search for potential antibiotic producing bacteria candidates. A serial dilution of the soil was performed and plated onto TSA plates for visual observation of bacteria. Any bacteria found to be producing zones of inhibition were subcultured onto master plates and also onto antibiotic screenings to be tested against safe relatives of ESKAPE pathogens. One specific candidate was found to inhibit the growth of several safe relative and was subcultured again to a quadrant streak plate. Polymerase Chain Reaction (PCR) analysis was performed in order to determine if gene sequencing was a viable option. This research was performed using the guidelines from Tiny Earth, a student-based program that seeks to find new antibiotic compounds from soil samples collected globally.
Image
Discovery and Analysis of Antibiotic Producing Bacteria from Locally Sourced Soil
CoLab
Locally sourced soil was extracted in order to search for potential antibiotic producing bacteria candidates. A serial dilution of the soil was performed and plated onto TSA plates for visual observation of bacteria. Any bacteria found to be producing zones of inhibition were subcultured onto master plates and also onto antibiotic screenings to be tested against safe relatives of ESKAPE pathogens. One specific candidate was found to inhibit the growth of several safe relative and was subcultured again to a quadrant streak plate. Polymerase Chain Reaction (PCR) analysis was performed in order to determine if gene sequencing was a viable option. This research was performed using the guidelines from Tiny Earth, a student-based program that seeks to find new antibiotic compounds from soil samples collected globally.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.