Searching for antibiotics in soil

Location

CoLab

Start Date

3-5-2019 12:00 PM

End Date

3-5-2019 1:15 PM

Document Type

Poster

Description

Bacterial pathogens are starting to develop a stronger resistance to antibiotics. With bacterial pathogens starting to develop a stronger antibiotic resistances, that makes it harder to treat patients with an infectious disease. Creating new antibiotics can be time consuming and costly which pharmaceutical companies are not willing to do. So with our experiment in lab, we tried to look for an organism in the soil that showed any signs of antibiotic production, I collected my soil sample near my house, and we serial diluted down to get countable colonies. We then tested bacteria from this soil sample against safe relatives of the ESKAPE pathogens to see if they would show signs of inhibition. After testing them against the safe relatives and seeing if any of them showed any signs of inhibition, we’re going to sequence the DNA by doing PCR and gel electrophoresis to see what my organism is and what antibiotic properties it possesses.

Comments

The faculty supervisor for this project was Jamie Cunningham, Biology.

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May 3rd, 12:00 PM May 3rd, 1:15 PM

Searching for antibiotics in soil

CoLab

Bacterial pathogens are starting to develop a stronger resistance to antibiotics. With bacterial pathogens starting to develop a stronger antibiotic resistances, that makes it harder to treat patients with an infectious disease. Creating new antibiotics can be time consuming and costly which pharmaceutical companies are not willing to do. So with our experiment in lab, we tried to look for an organism in the soil that showed any signs of antibiotic production, I collected my soil sample near my house, and we serial diluted down to get countable colonies. We then tested bacteria from this soil sample against safe relatives of the ESKAPE pathogens to see if they would show signs of inhibition. After testing them against the safe relatives and seeing if any of them showed any signs of inhibition, we’re going to sequence the DNA by doing PCR and gel electrophoresis to see what my organism is and what antibiotic properties it possesses.