Microbiology soil sample research project
Location
CoLab
Start Date
3-5-2019 1:30 PM
End Date
3-5-2019 2:45 PM
Document Type
Poster
Description
We are searching for antibiotic producing bacteria. We obtained numerous bacteria colonies from conducting serial dilutions from our soil sample. After choosing bacteria that appeared to have zones of inhibition, I created a master plate to isolate those colonies. We tested which our candidates would reproduce zones of inhibition with the ESKAPE safe relatives. My chosen candidate produced zones of inhibition on most of the ESKAPE safe relatives except Pseudomonas putida. Too further isolate my chosen candidate colonies; I created quadrant plates to have individual colonies of a single chosen candidate. To identify the genus of our bacteria, we did DNA sequencing testing, and metabolic testing as well.
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Microbiology soil sample research project
CoLab
We are searching for antibiotic producing bacteria. We obtained numerous bacteria colonies from conducting serial dilutions from our soil sample. After choosing bacteria that appeared to have zones of inhibition, I created a master plate to isolate those colonies. We tested which our candidates would reproduce zones of inhibition with the ESKAPE safe relatives. My chosen candidate produced zones of inhibition on most of the ESKAPE safe relatives except Pseudomonas putida. Too further isolate my chosen candidate colonies; I created quadrant plates to have individual colonies of a single chosen candidate. To identify the genus of our bacteria, we did DNA sequencing testing, and metabolic testing as well.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.