Searching for Antibiotic Producing Bacteria in Backyard Soil
Location
CoLab
Start Date
3-5-2019 1:30 PM
End Date
3-5-2019 2:45 PM
Document Type
Poster
Description
As time progresses and bacteria become more and more antibiotic resistant, it is important to continue the search for the next antibiotic producing microbes. This research looks at common backyard soil, and determines if it contains antibiotic producing microbes. This was done by taking the soil and using serial dilution to isolate bacterial colonies. These colonies were separated onto master plates, and then tested against the safe relatives of the ESKAPE pathogens using a lawn of growth technique. The candidate then underwent metabolic testing and PCR sequencing to classify the genus of the bacteria. The metabolic testing showed how and with what resources the bacteria survives and thrives. Ultimately, this research is important because pathogens are becoming resistant to the antibiotics we do have, and the next promising antibiotic producing bacteria could be living in backyard soil.
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Searching for Antibiotic Producing Bacteria in Backyard Soil
CoLab
As time progresses and bacteria become more and more antibiotic resistant, it is important to continue the search for the next antibiotic producing microbes. This research looks at common backyard soil, and determines if it contains antibiotic producing microbes. This was done by taking the soil and using serial dilution to isolate bacterial colonies. These colonies were separated onto master plates, and then tested against the safe relatives of the ESKAPE pathogens using a lawn of growth technique. The candidate then underwent metabolic testing and PCR sequencing to classify the genus of the bacteria. The metabolic testing showed how and with what resources the bacteria survives and thrives. Ultimately, this research is important because pathogens are becoming resistant to the antibiotics we do have, and the next promising antibiotic producing bacteria could be living in backyard soil.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.