Start Date
28-4-2022 1:30 PM
Document Type
Poster
Description
As antibiotics are used more frequently, antibiotic resistance becomes more prevalent which makes the study of soil microbiology necessary. When soil is gathered and studied there is a chance a new bacterium that produces antibiotic compounds could be discovered that show no resistance, solving this rising problem. To start this project, I first collected my soil sample (Salina, KS) and conducted a serial dilution on it. After incubation, I examined my plates and transferred potential candidates onto a master plate. I then challenged these candidates against safe relative ESKAPE pathogens; ES009, my focus candidate, produced halos around 5/6 of the safe relative ESKAPE pathogens. In hopes of gathering all the information possible, I conducted many stains (simple, smear, gram, spore, acid fast), created streak plates, and did a PCR. My candidate is gram negative, flat in elevation, has smooth borders, is off-white/creamy in color, opaque, and approximately 0.3 mm in size. Due to time limitations, the polymerase chain reaction (PCR), gel electrophoresis, and sequencing results are not available at this time but will be presented on my poster at the symposium.
Does My Soil Sample Contain Bacteria with Antibiotic Compounds?
As antibiotics are used more frequently, antibiotic resistance becomes more prevalent which makes the study of soil microbiology necessary. When soil is gathered and studied there is a chance a new bacterium that produces antibiotic compounds could be discovered that show no resistance, solving this rising problem. To start this project, I first collected my soil sample (Salina, KS) and conducted a serial dilution on it. After incubation, I examined my plates and transferred potential candidates onto a master plate. I then challenged these candidates against safe relative ESKAPE pathogens; ES009, my focus candidate, produced halos around 5/6 of the safe relative ESKAPE pathogens. In hopes of gathering all the information possible, I conducted many stains (simple, smear, gram, spore, acid fast), created streak plates, and did a PCR. My candidate is gram negative, flat in elevation, has smooth borders, is off-white/creamy in color, opaque, and approximately 0.3 mm in size. Due to time limitations, the polymerase chain reaction (PCR), gel electrophoresis, and sequencing results are not available at this time but will be presented on my poster at the symposium.
Comments
The faculty mentor for this project was Angela Consani, Biology.