Discovering an Antibiotic Producing Candidate
Location
CoLab
Start Date
3-5-2019 1:30 PM
End Date
3-5-2019 2:45 PM
Document Type
Poster
Description
The goal of this research project is to discover antibiotic producing bacteria. To begin the project, a soil sample was collected from De Soto, Kansas. The sample was then serially diluted in a test tube and then onto 50% TSA agar plates. Some of the plates produced zones of inhibition around select colonies. These colonies were used to create a master plate. From the master plate, each of the numbered bacteria (eleven total) was tested against six tester strains: P. putida, A. baylyi, E. faecalis, S. epidermidis, E. aerogenes, E. coli. From the tester strains, candidate #7 had the most potential because it was the only bacteria in which zones of inhibition appeared against all of the tester strains. Also, from the master plate again, a quadrant streak plate was created using candidate #7 to develop isolated colonies that will be used in further experiments. It was discovered that the candidate is Gram-negative. Additionally, metabolic testing and sequencing at the genus level will help to discover more about this potential antibiotic-producing candidate.
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Discovering an Antibiotic Producing Candidate
CoLab
The goal of this research project is to discover antibiotic producing bacteria. To begin the project, a soil sample was collected from De Soto, Kansas. The sample was then serially diluted in a test tube and then onto 50% TSA agar plates. Some of the plates produced zones of inhibition around select colonies. These colonies were used to create a master plate. From the master plate, each of the numbered bacteria (eleven total) was tested against six tester strains: P. putida, A. baylyi, E. faecalis, S. epidermidis, E. aerogenes, E. coli. From the tester strains, candidate #7 had the most potential because it was the only bacteria in which zones of inhibition appeared against all of the tester strains. Also, from the master plate again, a quadrant streak plate was created using candidate #7 to develop isolated colonies that will be used in further experiments. It was discovered that the candidate is Gram-negative. Additionally, metabolic testing and sequencing at the genus level will help to discover more about this potential antibiotic-producing candidate.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.