Searching for Antibiotic Producing Bacteria
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Location
CoLab
Start Date
3-5-2019 10:30 AM
End Date
3-5-2019 11:45 AM
Document Type
Poster
Description
The purpose of this project is to find antibiotic producing bacteria. Gather soil from an area of your choice and create 5 serial dilution tubes and plates with it. This process is necessary to find antibiotic producing colonies that had zones of inhibition around them. After closely searching the plates with denser growth, identify 12 potential candidates that formed zones of inhibition. Transfer these 12 colonies one by one aseptically to a numbered master plate. Do an antibiotic screening to see if any of the candidates can inhibit the growth of the 6 safe relatives of the ESKAPE pathogens. Next step in the experiment is to get a colony with a zone of inhibition isolated. Perform a quadrant streak to achieve that. After that, perform a Gram stain test to determine whether it was Gram negative or Gram positive. Under the microscope, it was clear to see it was pink, which meant Gram negative bacillus. Perform metabolic testing and sequencing to know at least the genus level of the organism.
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Searching for Antibiotic Producing Bacteria
CoLab
The purpose of this project is to find antibiotic producing bacteria. Gather soil from an area of your choice and create 5 serial dilution tubes and plates with it. This process is necessary to find antibiotic producing colonies that had zones of inhibition around them. After closely searching the plates with denser growth, identify 12 potential candidates that formed zones of inhibition. Transfer these 12 colonies one by one aseptically to a numbered master plate. Do an antibiotic screening to see if any of the candidates can inhibit the growth of the 6 safe relatives of the ESKAPE pathogens. Next step in the experiment is to get a colony with a zone of inhibition isolated. Perform a quadrant streak to achieve that. After that, perform a Gram stain test to determine whether it was Gram negative or Gram positive. Under the microscope, it was clear to see it was pink, which meant Gram negative bacillus. Perform metabolic testing and sequencing to know at least the genus level of the organism.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.