Microbiology Abstract
Location
CoLab, OCB 100
Start Date
27-4-2018 9:00 AM
Document Type
Poster
Description
There is a vast amount of existing antibiotics that the immune system is building or has a built a resistance to. It is necessary to find new ones to fight against bacterial infections in humans. Since the introduction and overuse of antibiotics we’ve seen a decline in their effectiveness. Cultivation of pure candidates for antibiotic producing activity were tested. This process involved diluting soil and looking-for colonies with zones of inhibition. After identifying my candidates, I created a master plate, the purpose of the master plate is to narrow down my candidates in search of the purest candidates that may yield the best results when tested against tester strains of different bacteria. Antibiotic screening was performed by testing candidates against gram-positive and gram-negative pathogens to see if those zones of inhibition appear. Sequencing as well as metabolic studies were performed to identify organisms.
Image
Microbiology Abstract
CoLab, OCB 100
There is a vast amount of existing antibiotics that the immune system is building or has a built a resistance to. It is necessary to find new ones to fight against bacterial infections in humans. Since the introduction and overuse of antibiotics we’ve seen a decline in their effectiveness. Cultivation of pure candidates for antibiotic producing activity were tested. This process involved diluting soil and looking-for colonies with zones of inhibition. After identifying my candidates, I created a master plate, the purpose of the master plate is to narrow down my candidates in search of the purest candidates that may yield the best results when tested against tester strains of different bacteria. Antibiotic screening was performed by testing candidates against gram-positive and gram-negative pathogens to see if those zones of inhibition appear. Sequencing as well as metabolic studies were performed to identify organisms.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.