Soil Bacteria: Testing for Antibiotic Activity
Location
CoLab, OCB 100
Start Date
27-4-2018 12:00 PM
Document Type
Poster
Description
With antibiotic resistant growing and evolving, and the lack of antibiotics to continue to treat highly resistant bacteria, the need for research in soil bacteria is a must. In lab, personal soil was brought to start testing for antibiotic properties. Starting with serial dilution, lab students were able to grow an array of bacteria on 50% TSA plates, find isolated colonies, removing colonies that had potential to be antibiotic producing, transferring those antibiotic producing colonies to obtain pure culture to test for antibiotic activity against both a gram-negative and gram-positive bacteria. Organism identity will be done by sequencing and metabolic tests to determine if a novel organism has been discovered.
Soil Bacteria: Testing for Antibiotic Activity
CoLab, OCB 100
With antibiotic resistant growing and evolving, and the lack of antibiotics to continue to treat highly resistant bacteria, the need for research in soil bacteria is a must. In lab, personal soil was brought to start testing for antibiotic properties. Starting with serial dilution, lab students were able to grow an array of bacteria on 50% TSA plates, find isolated colonies, removing colonies that had potential to be antibiotic producing, transferring those antibiotic producing colonies to obtain pure culture to test for antibiotic activity against both a gram-negative and gram-positive bacteria. Organism identity will be done by sequencing and metabolic tests to determine if a novel organism has been discovered.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.