Antibiotics in Flower Bed Soil?
Location
CoLab, OCB 100
Start Date
27-4-2018 12:00 PM
Document Type
Poster
Description
Antibiotic resistance is spreading faster than the development of new antibiotics. Antibiotic resistance is the pathogen’s ability to mutate and fight off the antibiotics. The increased usage of antibiotics is creating antibiotic resistance. Therefore, it is important to research and find new antibiotic-producing microorganisms that combat pathogens. Historically, most antibiotics were discovered by screening soil microorganisms. I began my project by collecting a loamy soil sample in a flower bed. I then diluted and distributed the soil sample on petri dishes to identify bacterial species. I chose bacterial candidates that might have antibiotic properties. I chose these bacterial candidates based on if they showed a zone of inhibition, (which is an area where a microorganism stopped certain bacteria from growing) which shows antibiotic properties. I tested each candidate by streaking them in their own petri dishes vertically and then streaking the safe tester strains horizontally. I did this to see if there was antibiotic properties in the candidates against those safe tester strains. I used safe tester strains because I did not want to test their relative ESKAPE pathogens. The ESKAPE pathogens are contagious and can cause disease unlike the safe tester strains that I used. First, I will be presenting my process of this experiment. Secondly, I will be presenting the results of the characterization and identification of my candidate bacterium. Finally, I will present the results on whether or not I have found antibiotic properties in my bacteria that I have grown.
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Antibiotics in Flower Bed Soil?
CoLab, OCB 100
Antibiotic resistance is spreading faster than the development of new antibiotics. Antibiotic resistance is the pathogen’s ability to mutate and fight off the antibiotics. The increased usage of antibiotics is creating antibiotic resistance. Therefore, it is important to research and find new antibiotic-producing microorganisms that combat pathogens. Historically, most antibiotics were discovered by screening soil microorganisms. I began my project by collecting a loamy soil sample in a flower bed. I then diluted and distributed the soil sample on petri dishes to identify bacterial species. I chose bacterial candidates that might have antibiotic properties. I chose these bacterial candidates based on if they showed a zone of inhibition, (which is an area where a microorganism stopped certain bacteria from growing) which shows antibiotic properties. I tested each candidate by streaking them in their own petri dishes vertically and then streaking the safe tester strains horizontally. I did this to see if there was antibiotic properties in the candidates against those safe tester strains. I used safe tester strains because I did not want to test their relative ESKAPE pathogens. The ESKAPE pathogens are contagious and can cause disease unlike the safe tester strains that I used. First, I will be presenting my process of this experiment. Secondly, I will be presenting the results of the characterization and identification of my candidate bacterium. Finally, I will present the results on whether or not I have found antibiotic properties in my bacteria that I have grown.
Comments
The faculty supervisor for this project was Jon Kniss.