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Location

CoLab

Start Date

3-5-2019 12:00 PM

End Date

3-5-2019 1:15 PM

Document Type

Poster

Description

Most antibiotics have been produced by screening soil microorganisms which has staved off since the 1960s. Approximately 99% of bacteria in soil and the environment has remained uncultured (L.L. Ling et al) and thorough consideration from the scientific community of discovery and cultivation of microorganisms in soil should be strongly adhered to in the coming years. By diluting, screening, and culturing my collected sample of soil, I isolated a strain of bacteria. After culturing to isolate colonies I created a quadrant streak with a purpose of having the correct range of bacteria to use to test that my strain to see if it proves to be antibiotic producing against our safe ESKAPE pathogens. After my strain showed to be antibiotic producing to Pseudomonas putida and Escherichia coli it was stained, and showed to be a Gram-positive, short-rod bacillus. PCR sequencing showed that it contained the target 16srRNA gene.

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May 3rd, 12:00 PM May 3rd, 1:15 PM

Digging For New Antibiotics

CoLab

Most antibiotics have been produced by screening soil microorganisms which has staved off since the 1960s. Approximately 99% of bacteria in soil and the environment has remained uncultured (L.L. Ling et al) and thorough consideration from the scientific community of discovery and cultivation of microorganisms in soil should be strongly adhered to in the coming years. By diluting, screening, and culturing my collected sample of soil, I isolated a strain of bacteria. After culturing to isolate colonies I created a quadrant streak with a purpose of having the correct range of bacteria to use to test that my strain to see if it proves to be antibiotic producing against our safe ESKAPE pathogens. After my strain showed to be antibiotic producing to Pseudomonas putida and Escherichia coli it was stained, and showed to be a Gram-positive, short-rod bacillus. PCR sequencing showed that it contained the target 16srRNA gene.