Event Title

Are There Any Antibiotic Producing Bacteria in the Baker Wetlands?

Location

CoLab

Start Date

3-5-2019 12:00 PM

End Date

3-5-2019 1:15 PM

Document Type

Poster

Description

In this experiment, I am seeking to examine whether or not there are any antibiotic producing bacteria found in my soil composition. I collected my soil sample from the Baker Wetlands in Lawrence, Kansas. The soil was about 100-200 meters away from a middle-sized pond. I first concentrated my soil sample by performing serial dilutions on five different Petri dishes to obtain plates of high concentrated bacteria and low concentrated bacteria. After those were incubated, I chose twelve single colonies of bacteria and made master plates with the twelve candidates. I then tested my twelve candidates against the safe ESKAPE pathogen bacteria using the lawn of growth method. Out of my twelve candidates, one of my bacteria showed a zone of inhibition against Staphylococcus epidermis. I then made a quadrant streak of that bacteria to isolate and form a pure colony. I have not done these yet, but the last two steps I will perform are metabolic testing and sequencing of my bacteria to find at least the genus level of the organism.

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May 3rd, 12:00 PM May 3rd, 1:15 PM

Are There Any Antibiotic Producing Bacteria in the Baker Wetlands?

CoLab

In this experiment, I am seeking to examine whether or not there are any antibiotic producing bacteria found in my soil composition. I collected my soil sample from the Baker Wetlands in Lawrence, Kansas. The soil was about 100-200 meters away from a middle-sized pond. I first concentrated my soil sample by performing serial dilutions on five different Petri dishes to obtain plates of high concentrated bacteria and low concentrated bacteria. After those were incubated, I chose twelve single colonies of bacteria and made master plates with the twelve candidates. I then tested my twelve candidates against the safe ESKAPE pathogen bacteria using the lawn of growth method. Out of my twelve candidates, one of my bacteria showed a zone of inhibition against Staphylococcus epidermis. I then made a quadrant streak of that bacteria to isolate and form a pure colony. I have not done these yet, but the last two steps I will perform are metabolic testing and sequencing of my bacteria to find at least the genus level of the organism.