Event Title

Discovery of Antibiotic Producing Bacteria

Location

CoLab

Start Date

3-5-2019 1:30 PM

End Date

3-5-2019 2:45 PM

Document Type

Poster

Description

This research project was performed to search for bacteria that produces antibiotics. The candidate bacteria was first isolated by performing serial dilutions and creating master plates of candidate bacteria. They were tested against the safe relatives of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa and the various species of Enterobacter) which are Enterococcus faecalis (E. faecalis), Staphylococcus epidermidis (S. epidermidis), Escherichia coli (E. coli), Acinetobacter baylyi (A. baylyi), Pseudomonas putida (P. putida) and Enterobacter aerogenes (E. aerogenes)to determine which strains are inhibited by the candidate. The candidate had zones of inhibition when tested against P. putida, S. epidermidis and E. coli. A Gram stain done on the candidate determined that the candidate is a Gram-negative bacillus bacteria. Polymerase Chain Reaction and gene sequencing was then performed on the DNA of the candidate to determine which genus it belongs to.

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May 3rd, 1:30 PM May 3rd, 2:45 PM

Discovery of Antibiotic Producing Bacteria

CoLab

This research project was performed to search for bacteria that produces antibiotics. The candidate bacteria was first isolated by performing serial dilutions and creating master plates of candidate bacteria. They were tested against the safe relatives of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa and the various species of Enterobacter) which are Enterococcus faecalis (E. faecalis), Staphylococcus epidermidis (S. epidermidis), Escherichia coli (E. coli), Acinetobacter baylyi (A. baylyi), Pseudomonas putida (P. putida) and Enterobacter aerogenes (E. aerogenes)to determine which strains are inhibited by the candidate. The candidate had zones of inhibition when tested against P. putida, S. epidermidis and E. coli. A Gram stain done on the candidate determined that the candidate is a Gram-negative bacillus bacteria. Polymerase Chain Reaction and gene sequencing was then performed on the DNA of the candidate to determine which genus it belongs to.